Based on the model recommended in the Global Action Plan and Guidelines, this National Action Plan on Anti-Microbial Resistance (NAPAMR) has been developed. Technical informants in different areas of work gathered local data on current initiatives. Using the policy structure established by the One Health policy, these were analysed. Interventions have been designed to resolve the gaps in all five of the global Action Plan's strategic objectives. Further consultations were conducted to ensure that the proposed measures in the affected sectors were feasible, valid and applicable within the Rwandan context.