Rwanda embraced One Health in 2011 and many activities have been implemented under this framework up to date. A One Health Steering Committee (now called the One Health Multi-Sectoral Coordination Mechanism – OH-MCM) was set up to lead and advise on matters related to One Health. Through this mechanism, the first national One Health Strategic Plan (ROHSP 2014-2018) was developed and several joint activities like zoonotic disease prioritization including development of preparedness and response plans for three of the six priority zoonotic diseases i.e. RVF, HPAI and Rabies were carried out. Areas of multi-sectoral collaboration for zoonotic disease surveillance, outbreak investigation and control were identified. The One Health Steering Committee was assessed using the One Health Planning for Performance tool (OH-P4P) and actions to improved its efficiency identified and discussed.