The Second Rwanda One Health Strategic Plan 2021-2026 (ROHSP II) therefore, comes to provide and re-assure partners but also the stakeholders of the re-commitment of all sectors to One Health. This Strategic Plan will bring to the fore its commitments and priorities for the coming five years and has taken into consideration what was done and what was not done in ROHSP I as well as capture and integrate potential One Health activities proposed in the HSSP IV and PSTA IV, Priority Zoonotic Diseases (PZDs) and the Joint External Evaluation (JEE) as well as the Performance of Veterinary Services (PVS) and the PVS -Gap analysis reports.