This is the first “National One Health Strategic Plan” to be developed using a multisectoral approach and has drawn expertise from various sectors reflecting shared commitment to enhanced collaboration among animal, wildlife and human health sectors to reduce the burden of zoonotic diseases. The coordination of the development of this plan was done by the Prime Minister’s Office of the United Republic of Tanzania. This strategic plan is a whole-of-government guiding document aimed at summarizing operations and activities among various stakeholders. This strategic plan also aims to create and maintain active collaboration between the sectors for the prevention and control of zoonotic diseases to ensure that there is timely preparedness, and a consistent and coordinated response in the event of an occurrence of a zoonotic event. To achieve this, the plan proposes the establishment of a “National Coordinating Unit” which will continue to evolve, enhance, and refocus One Health programs to meet existing and impending challenges. To support the unit, a One Health Steering Committee and technical working groups will be created.